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Objective:To assess the left ventricular myocardial function in non-ST-segment-elevation acute coronary syndrome (NSTE-ACS) patients with normal wall motion and left ventricular ejection fraction (LVEF) after percutaneous coronary intervention(PCI) by noninvasive myocardial work technology, and to explore the evolution of left ventricular myocardial function recovery.Methods:A total of 92 NSTE-ACS patients from July to December 2019 in Beijing Chao Yang Hospital with normal wall motion and LVEF (>55%) after PCI were recruited. Echocardiography was performed 1 day before PCI, 1 day, 2 weeks, 1 month, and 3 months after PCI. Global longitudinal strain (GLS) was analyzed, and Brachial cuff systolic pressure was used as left ventricular pressure to construct a non-invasive left ventricular pressure-strain loop. Global myocardial work index (GWI), global constructive work (GCW), global waste work (GWW), global myocardial work efficiency (GWE) among groups were compared and their correlations with strain parameters were explored.Results:GWI, GCW, GWE were improved ( P<0.05) at 1 day after PCI, GLS improved ( P<0.05) and GWW decreased ( P<0.05) at 2 weeks, LVEF improved ( P<0.05) at 1 month. Baseline GWI and GCW had a moderately negative correlation with GLS ( r=-0.67, -0.66; both P<0.05); GWW had a moderately positive correlation with mechanical dispersion(MD) and postsystolic shortening index(PSI) ( rs=0.45, 0.50; both P<0.05); GWE had a moderately negative correlation with GLS, MD and PSI ( rs=-0.47, -0.55, -0.56; all P<0.05). Conclusions:Left ventricular myocardial function gradually improves in NSTE-ACS patients with normal wall motion and LVEF after PCI. Myocardial work parameters changes are more sensitive than GLS and LVEF, and can assess early left ventricular myocardial function changes after PCI.
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Objective:To assess global myocardial work in non-ST-segment-elevation acute coronary syndrome (NSTE-ACS) patients with no obvious regional wall motion abnormalities and preserved left ventricular ejection fraction (LVEF) by noninvasive left ventricular (LV) pressure-strain loops, and to explore its diagnostic value in patients with NSTE-ACS.Methods:A total of 169 NSTE-ACS patients with normal wall motion abnormalities and LVEF (>55%) were recruited in Beijing Chao Yang Hospital, Capital Medical University from June to December 2019. The patients were divided into two groups according to the degree of coronary stenosis, including severe coronary artery stenosis group ( n=121), and no severe stenosis group ( n=48). The patients of severe coronary artery stenosis group were further subdivided into single-vessel severe stenosis group ( n=52) and multi-vessel severe stenosis group ( n=69). Global longitudinal strain (GLS) analysis was performed by speckle tracking echocardiography before coronary angiography. Brachial cuff systolic pressure was used as left ventricular pressure to construct a non-invasive left ventricular pressure-strain loop. Global myocardial work index (GWI), global constructive work (GCW), global waste work (GWW) and global myocardial work efficiency (GWE) was computed by LV pressure-strain loops with a proprietary algorithm between groups. ROC curve analysis was used to determine the optimal cutoff value of the parameters to detect severe coronary artery stenosis. Independent factors affecting left ventricular myocardial function were assessed by Logistic regression analysis. Results:GLS, GWI, GCW and GWE were significantly reduced, and GWW was increased in severe coronary artery stenosis group than in no severe stenosis group(all P<0.05). GLS was significantly reduced in multi-vessel severe stenosis group ( P<0.05) but not in single-vessel severe stenosis group ( P=0.32). GWE was an independent factor affecting myocardial function in severe coronary artery stenosis group, GWE<96% had a area under the curve (AUC)=0.83 (74% for sensitivity, 81% for specificity) to identify severe coronary artery stenosis, and was superior to GLS (AUC=0.66, P<0.05) and GWI (AUC=0.70, P<0.05). Conclusions:In NSTE-ACS patients with severe coronary artery stenosis, no obvious regional wall motion abnormalities and preserved LVEF, LV global myocardial function is impaired based on noninvasive pressure-strain loops, GWI, GCW, and GWE are reduced, and GWW is increased, and GWE is a more sensitive index than GLS and GWI to predict severe coronary artery stenosis in NSTE-ACS patients.
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To investigate the association between the change of left ventricular ( LV ) function and mechanical dispersion ( MD ) and exercise capacity in patients with hypertrophic cardiomyopathy ( HCM ) by exercise stress echocardiography . Methods Sixty‐five HCM patients [ 40 cases of hypertrophic non‐obstructive cardiomyopathy ( HNCM ) , 25 cases of hypertrophic obstructive cardiomyopathy ( HOCM ) ] and 25 control subjects were recruited .LV function ,MD and exercise capacity were evaluated by two‐dimensional speckle‐tracking imaging and echocardiography at rest and during exercise ,and the following parameters of LV function were recorded : LV global longitudinal strain ( LVGLS) ,MD ,early diastolic strain rate ( Sre) ,the ratio of peak early diastolic mitral inflow and annulus velocity ( E/e′) ,LV outflow tract gradient ( LVO TG) ; LV functional reserve was assessed by ΔLVGLS and ΔSRe ; exercise capacity was evaluated by metabolic equivalents ( M ET s ) . T he association between the change of LV function and MD and exercise capacity was investigated . Results ①Compared with normal controls ,LVO TG ,E/e′ and MD increased ,and LVGLS ,Sre , ΔLVGLS , ΔSRe and M ET s decreased in HNCM patients at rest and during exercise ( all P ＜ 0 .05 ) . ② LVO TG , E/e′ and MD were further increased ,LVLGS ,Sre ,ΔSRe and M Ets were further reduced in HOCM patients compared with HNCM patients ( all P ＜ 0 .05 ) . ③LVGLS and MD measured at peak exercise were associated with M ET s ( r ＝－0 .68 , P ＜ 0 .001 ; r ＝ －0 .43 , P ＜ 0 .001 ) . ④ ROC curve analysis showed LVGLS had a better predictive value for exercise intolerance in HCM patients ,followed by E/e′ and MD . Conclusions LV function and mechanic reserve are reduced but MD is increased in HCM patients ,especially in HOCM patients . Exercise capacity is associated with LV function and MD ,w hich can predict the reduced exercise capacity in HCM patients .
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Objective To explore the clinical feature,treatment and prognosis of incidental prostate cancer(IPC) after transurethral resection of the prostate (TURP) for benign prostatic hyperplasia (BPH).Methods From January 2009 to April 2017,24 cases undergoing TURP for benign prostatic hyperplasia and being diagnosed with prostate cancer(T1a-T1b) was retrospectivey analysed,who aged from 62 to 84 years (mean 71.8 years).Digital rectal examination (DRE) showed prostate medium texture,smooth surface,and no nodules.Ultrasound presented no low echo nodules in the prostate.Prostate volumes were 19.2-93.4 ml,with median of 40.1 ml.PSA were 1.81-9.11 ng/ml,with median of 4.12 ng/ml.The patients with PSA between 6-10 ng/ml accepted prostate biopsy,and pathological results were negative.Results The The pathology of TURP specimens in 24 cases were diagnosed prostate cancer (21 cases of T1a,3 cases of T1b).According to the new WHO/ISUP classification group,there were 18 cases of hierarchical group 1,3 cases of hierarchical group 2,1 case of hierarchical group 3,2 cases in hierarchical group 4.All patients were treated with hormonal therapy,and 7 cases (5 cases of hierarchical group 1,and 2 cases of hierarchical group 2) underwent laparoscopic radical prostatectomy (LRP) after 3 months of hormonal therapy.The specimens of prostatectomy were examined by whole-mount serial,showing 3 cases of prostate cancer (T1a) with negative margin,and 4 cases of benign prostate cells.They were followed up for 5-82 months with median of 43.5 months.No biological progression or tumor progression was found,and,1 case died of colon cancer after 26 months of follow-up.The patients' age and Gleason score of stage T1b were higher than that of stage T1a.Prostate volume and preoperative PSA had no statistically significant difference between the two stages.Conclusions The patients' age and Gleason score of stage T1b were higher than that of stage T1b.The proportion of residual tumor following TURP was high.The prognosis of incidental prostate cancer was good by hormonal therapy or radical prostatectomy.
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Objective To evaluate right ventricular ( RV ) function in male rats with pulmonary arterial hypertension (PAH) induced by Monocrotaline (MCT) and discuss the effect of 17β-estradiol (E2) on RV function in PAH rats . Methods Thirty-two male SD rats were randomly divided into 4 groups( n =8) :MCT group ,MCT+E2 group ,MCT+Veh group and control group . MCT group ,MCT +E2 group and MCT+Veh group were given monocrotaline through intraperitoneal injection . MCT + E2 group and MCT+Veh group were also treated with E2 and placebo at the same time respectively . Echocardiography was performed four weeks later after MCT injection . Right heart catheterization was performed after echocardiography . Echocardiography right ventricular function parameters included:tricuspid annular plane systolic excursion (TAPSE) ,RV index of myocardial performance (RIMP) ,right ventricular fractional area change ( RVFAC) ,tissue Doppler-derived tricuspid lateral annular systolic velocity ( s′) and right ventricular free wall longitudinal strain ( RVLSFW ) . Results Compared with control group ,TAPSE ,RVFAC and RVLSFW decreased ,and RIMP increased in MCT group ,( P <0 .001 , P <0 .001 , P <0 .001 , P =0 .001 , P <0 .001 ,respectively) . TAPSE ,RVFAC and RVLSFW in MCT+ E2 group were higher and RIMP was lower than those in MCT group ( P < 0 .001 , P = 0 .001 , P = 0 .001 , P = 0 .002 , P = 0 .002 , respectively) . The parameters regarding RV function were slightly lower in MCT + E2 group ,compared with control group . However , these differences had no obvious statistical significance ( P > 0 .05 ) . Moreover ,Pearson correlation analysis showed that the serum E2 level was positively correlated with TAPSE ,RVFAC ,s′and RVLSFW ( r =0 .845 , P <0 .001 ;r =0 .859 , P <0 .001 ;r =0 .802 , P =0 .006 , respectively) ,and negatively correlated with RIPM ( r = -0 .803 , P <0 .001) . Conclusions RV function in male PAH rats induced by MCT decreased significantly ,while E2 could improve RV function in PAH rats and has a protective effect on RV function . Echocardiography could contribute to the quantitative evaluation of RV function in PAH rats induced by MCT .
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Objective: To explore the clinical value of plasma copeptin level on major adverse cardiovascular event (MACE) occurrence in patients with acute ST-segment elevation myocardial infarction (STEMI) during hospitalization. Methods: Our research included 2 groups:STEMI group, n=80 and Control group, n=80 patients with stable coronary artery disease (CAD). All patients were treated in our hospital from 2012-06 to 2014-06. Plasma level of copeptin was detected by ELISA, other relevant examinations were conducted to study the MACE occurrence in STEMI patients. Results: Plasma copeptin level in STEMI group (523.26 ± 142.69) pg/ml was higher than that in Control group (345.25 ± 89.36) pg/ml, P Conclusion: Plasma copeptin level could effectively predict MACE occurrence in patients with acute STEMI during hospitalization, it may predict their prognosis at certain point.
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Objective To evaluate the effect of As2O3 combined with paclitaxel(PTX)on the treatment of lung cancer.Methods The anti-proliferation efficiency of As2 O3 combined with PTX was evaluated by MTT assay.Tumor spheroids were used to evaluate anti-tumor ability of As2 O3 combined with PTX.Transmission electron microscope (TEM)were used to observe the apoptosis morphous.A549 cell were xenografted in mice to establish the animal model,and the nude mices were devided into four groups,saline group,As2 O3 group,PTX group and As2 O3 +PTX group.The animal model were used to evaluate the effect of anti-tumor.The tumor size of every group were measured.HE was used to observe the apoptosis of cancer cells. Results The cell inhibition rate of A549 cell were(3.35 ±0.21)%,(47.55 ±2.25)%,(64.64 ±3.35)%and(84.58 ±3.76)%after treatment with saline,As2O3,PTX and As2O3combined with PTX after 48h respectively(P<0.01).The early apoptosis rate of cancer cells were 0.26%,9.7%, 17.8% and 42.5% for saline group,As2 O3 group,PTX and As2 O3 +PTX group respectively(P<0.01 ).The final tumor spheroid volumes in saline group increased 1.36 times after 7 days.The final tumor spheroid volumes reduced to(77.35 ±2.31)%,(61.68 ±2.44)% and(44.85 ±3.34)% in As2O3,PTX and As2O3 combined with PTX group respectively(P<0.01).The inhibition of lung cancer in vitro demonstrated the inhibition rate of tumor growth compared with saline group were(22.4 ±4.5)%,(39.5 ±6.2)% and(69.5 ±7.3)% for As2O3,PTX and As2O3 +PTX,respectively(P<0.01 ).Conclusion As2 O3 combined with PTX can effectively inhibit the proliferation of A549 cells and ectopic tumor growth in nude mice and it may be a potentially effective treatment for lung cancer.
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Objective To compare the effect of retroperitoneoscopic ureterolithotomy (RPUL) and ureteroscopic lithotripsy (URL) for upper ureteral calculi.Methods One hundred and twenty cases treated by RPUL and 108 cases by URL from January 2002 to October 2012 were reviewed.In RPUL and URL group,the diameter of stone was (1.56 ± 0.52) cm vs (1.44 ± 0.46) cm,ipsilateral hydronephrosis was (2.85 ± 0.86) cm vs (2.76 ± 0.82) cm,body mass index was (23.65 ± 2.80) kg/m2 vs (22.54 ± 2.68) kg/m2.There were no signficant differences.Data on the operation time,the hospital stay after operation,the operation,successsful rate,complication incidence and stone-free rate were compared between the 2 groups.Results Comparisons between RPUL group and URL group included the following:the operation time was (75.5 ± 25.8) min vs (62.5 ± 15.3) min,the hospital stay after operation was (6.2 ± 1.2) d vs (4.0 ± 0.8) d.There were significant differences.The operation successful rate was 95.0％ (114/120) in RPUL group and 85.2％ (92/108) in URL group.The complications incidence rate was 3.5％ (4/114) in RPUL group and 17.4％ (16/92) in URL group.The stone-free rate was 100.0％ (114/114) in RPUL group and 89.1％ (82/92) in URL group.The differences were significant (P ＜ 0.05).Conclusions RPUL and URL had the advantages of less trauma and blood loss and rapid recovery.RPUL had fewer complication and higher success rate than URL,and could be a minimally invasive option for the treatment of ureteral calculi.
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Objcetive: To investigate the effect of resveratrol (RES)combined with cisplatin (DDP)on the cholangiocarcinoma cell line FRH-0201 in vitro. Methods: FRH-0201 cells were exposed to different concentrations of RES or (and)DDP. Cell morphological changes were observed by light and fluorescence microscopy. Inhibition of cell proliferation was measured by MTT and colony-forming assay. And the flow cytometry were performed to detect the cell apoptosis rate and cell cycle. Results: Resveratrol inhibited the proliferation of FRH-0201 cells in a time-and dose-dependent manner in the concentration of 5～320 ?mol/L and the IC50 values after the exposure of 24, 48, and 72 h were 55.35, 32.84, and 28.01 ?mol/L respectively. RES and DDP had synergetic effect in the inhibition of FRH-0201 cells. Conclusion: RES can inhibit the growth of FRH-0201 cells and has the synergetic effect with DDP.
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Objective To investigate the value of modified pancreatoduodenectomy in the treatment of ampullary carcinoma by comparing the efficacy of pancreatoduodenectomy with preservation of ascending portion of duodenum and that of Whipple procedure. Methods The clinical data of 133 patients with ampullary carcinoma who had been admitted to the Qilu Hospital from 1990 to 2006 were retrospectively analyzed. Of the 133 patients, 88 received Whipple procedure (group A) and 45 received pancreatoduedenectomy with preservation of ascending portion of duodenum (group B). The preoperative condition of the patients and short- and long-term efficacy of the 2 procedures were compared by Fisher exact probability, t test, chi-square test, Kaplan-Meier survival curve and Log-rank test, and the differences between the efficacy of the 2 procedures were compared. Results The operation time, time of gastrointestinal function recovery, hospital stay and ratio of patients with blood transfusion were (366±111) minutes, (7.4±1.4) days, (24±9) days and 76% (67/88) in group A, and (325±32)minutes, (4.3±1.4)days, (31±14)days, 42% (19/45) in group B, with significant difference between the 2 groups (t = 2.34, 2.08, 1.98 ; χ2 = 14.99, P < 0.05). The medium survival time of patients in groups A and B were (19.0±1.5)months and (16.9±1.7) months, with no significant difference between the 2 groups (χ2 = 0.46, P > 0.05). There was no significant difference in the occurrence of postoperative complications between the 2 groups (P > 0.05). Conclusions Pancreateduodenectomy with preservation of ascending portion of duodenum can be applied for patients with ampullary carcinoma.
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Objective To evaluate the diagnosis and treatment of cystic renal cell carcinoma and to improve the preoperative diagnosis and curative rate of the disease. Methods Ten cases of cystic renal cell carcinoma were retrospectively analyzed in the aspects of imaging and pathologic characteristics. There were 7 males and 3 females with average age of 56 years old (ranging from 38--74 years old) in this study. There were 3 cases complained of sore waist, 7 cases were found renal masses in annual physical examination and 2 cases had the history of renal cysts. The cyst diameter was 3.5 8.2 cm. Six cases had been diagnosed with ultrasound and 7 cases had been diagnosed with CT scan pre-operatively. Eight eases were diagnosed with frozen section during operation. All the 10 cases accepted radical nephreetomies. Results The post-operative histological diagnosis showed that there were 9 cases of clear cell carcinoma and 1 case of granular cell carcinoma. The pathological character istics were tumor necrosis of renal cell carcinoma in 6 cases, multilocular cystic renal cell carcinoma in 2 cases and carcinoma in renal cyst in 2 cases. Eight patients followed up from 6 months to 5 years. Six patients were still alive (mean 28.5 months). Conclusion The keys to improve the diagnosis and curative rate of the cystic renal cell carcinoma are paying attention to the pre-operative imaging study, the intra-operative frozen section examination and histopathology results.
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Objective To investigate the effect of antisense oligodeoxynueleotide targeting sur-vivin (survivin ASODN) on hilar cholangioearcinoma cell line FRH-0201 depressing the expression of survivin. Methods Survivin ASODN was transfected into hilar eholangiocarcinoma cell line FRH-0201by liposome. Morphologieal changes were observed by inverted phase contrast microscope. RT-PCR and Western blot methods were performed to detect the expressions of survivin mRNA and protein re-spectively. The changes in cell apoptosis were detected by flow cytometry. Results The expression of survivin mRNA and protein was significantly decreased in survivin ASODN group than that in the con-trol group(mRNA: 0.51-t-0. 03 vs 0. 82-t-0.02,P%0. 05～protein: 1.82-t-0.16 vs 3. 08--t-_0. 27, P-Q 0.05). The morphologieal apoptotic changes were observed and the apoptosis rate was increased (11.50+1.49% vs 0.39-+-0.08～, P%0.05). Conclusion Survivin AS()DN can induce hilar cholan-gioeareinoma cell line FRH-0201 into apoptosis by decreasing the expression of survivin.
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<p><b>OBJECTIVE</b>To establish a new extrahepatic growing hepatocellular carcinoma cell line.</p><p><b>METHODS</b>A specimen from extrahepatic growing hepatocellular carcinoma was cultured in vitro. Cancer cells were studied morphologically and subjected to karyotype analysis, DNA analysis, and tumor formation evaluation.</p><p><b>RESULTS</b>Morphological observation and functional analysis showed that their features were similar to those of HCC. Chromosomes with a variation of 76 approximately 104 were able to secret AFP in vitro and to form bile canaliculi with microvilli.</p><p><b>CONCLUSION</b>EGHC-9901 cell line has characteristics of the extrahepatic growing hepatocellular carcinoma.</p>
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Adult , Animals , Humans , Male , Mice , Carcinoma, Hepatocellular , Genetics , Pathology , Chromosome Aberrations , Liver Neoplasms , Genetics , Pathology , Mice, Nude , Tumor Cells, Cultured , alpha-FetoproteinsABSTRACT
Objective: To evaluate the rationality of the use of antibiotic prophylaxis in the perioperative period of herniorrhaphy. Methods: Seventy-five cases of herniorrhaphy discharged in March, June and September of 2009 were collected. Parameters including indications, the types of antibacterial drugs, time of administration, solvent choice, usage and dosage, combination, etc, were assessed for the rationality according to the "the guiding principles of clinical use of antibacterial drugs"and other relevant documents. Results: The antibacterial drugs application rate was 100%, while the rationali-ty of the drug selection, the drug administration route, the single dose and the number of dosing, was 70.7%, 100% , 100% and 93.3% respectively. Sixty-two patients(82.7%)were treated with antibacterial drugs at 0.5～2 hours before surgery, and the average time course of the prophylactic use of antibiotics after surgery was 2.3 days. Conclusions: Some problems existed for the use of antibiotic prophylaxis in the perioperative period of herniorrhaphy. Surgeon should ensure the correct use of the antibacterial drugs to make the safety, efficiency, rationality, and economy for the patients.
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Objective: To investigate the effect of vascular endothelial growth factor-C (VEGF-C) on invasive capability and proliferation of human cholangiocarcinoma cells. Methods: The effect of VEGF-C on FRH0201 was assayed by MTT. The cycle pattern and apoptosis was assayed using flow cytometry. The effect of VEGF-C on homotypic adhesion and metastasis in FRH0201 was examined with 3H-TdR infiltration and Boyden chamber. Results: VEGF-C could enhance the proliferation of FRH0201 in a dose and time dependent manner. And VEGF-C could inhibit cell apoptosis significantly. After cultured for 2 hours with 1, 5, 10ng/ml of VEGF-C, there were more cells in the lower chamber than the control group. After 60, 90, 120 minutes induction by 1ng/ml、5ng/ml、10ng/ml, the cells showed significantly lower homotypic adhesion than that of the control group. Conclusions: VEGF-C could enhance the proliferation of FRH0201 and inhibit cell apoptosis. VEGF-C could decrease homotypic adhesion of FRH0201 and might be a cause of the metastasis.
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10 days.All the patients received operations.Results:The prognoses of 80.49% patients in the early and middle stages were well ,the fadeaway of edema was evident,on the contrary,the prognoses of 83.33% patients of the later stage were worse,the fadeaway of edema was slow.Conclusion:The patients of LEDVT should receive operation as early as possibly in 10 days,the patients should receive conservative therapy after 10 days.
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Objective: To study the effect of hyperthermia treatment on apoptosis of human gallbladder carcinoma cell line GBC-SD.Methods: Human cultured gallbladder carcinoma cell GBC-SD in vitro was heated for 1 hour at 40℃ and 43℃.Then 6h、12h、20h later GBC-SD cells were collected,respectively.The morphologic character was observed under the microscope,the biological character was ascertained by DNA electrophoresis,the percentage of the cell apoptosis and the alteration of cell cycle were detected by flow cytometry.Results: After 1 hour heated at 43℃,gallbladder carcinoma GBC-SD cells occurred the most obvious apoptosis in 12h and 20h.Typical apoptotic bodies appeared,and DNA Ladder was demonstrated on DNA electrophoresis.Apoptotic peak and the change of cell cycle were tested by the flow cytometry.Conclusion:One hour moderate hyperthermia treatment at 43℃ is the effective heat-dosage to induce human gallbladder carcinoma GBC-SD cell apoptosis. [
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To establish a new extrahepatic growing hepatocellular carclnoma cell line. Methods: A speciment that was pathologically identified extrahepatic growing hepatocellular carcinoma were cultured in vitro. The morphology,karyotype analysis, DNA analysis, the tumor formation of heterotransplantation were observed. Results: Morphological observation and functional analysis showed that it had the comnnon features of HOC.It was found to be able to secret AFP in vitro. Conclusion: EGHC-9901 has characteristic of the extrahepatic growing hepatocellular carcinoma cell line.
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Objective : The patients with inguinal hernia were treated with plug mesh hernia repair. Methods:The hernia sacs were isolated and dissected back to the internal ring. The unoperated sacs were allowed to drop back through the internal ring into the abdominal cavity. A cone-shaped mesh hernia plug is inserted tapered end through ring and placed into position just beneath the crura. All our repairs were reinforced a second piece of flat was placed from the pubic tubercle, overlying the direct space. Results : Com-paired with conventional suture surgical techniques, a plug repair uses less disscection and ensures tenssion free hernioplusty. Conclusion : We believe that the two factors are the most important reseasons for greater patients confort, rapit rehabilitation, decreased recurrence and lessened overall complication rates with the mesh hernia plug techniques.
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Objective:To determine whether alpha-fetoprotein(AFP) messenger RNA(mRNA),a specifically hepatocyte-expressed gene,could be a marker of metastasis in hepatocellular carcinoma. Methods:Peripheral blood samples were obtained from 30 patients with hepatocellular carcinoma (HCC).A sensitive reverse transcription polymerase chain reaction( RT-PCR) technique was used to detect the human AFP mRNA in the blood.Results:Of the 30 HCC patients,14(46.7%) patients had positive AFP mRNA.The presence of AFP mRNA correlated with the TNM clinical stages,the size of tumor,extrahepatic metastasis or with portal vein carcinothrombosis(P0.05).Conclusion:The positive of AFP mRNA in the peripheral blood can indicate the presence of hematogenous metastasis in patients with HCC.